Overview

  • Product name
  • Description
    Rabbit polyclonal to MURF1
  • Specificity
    ab183094 detects endogenous level of total MURF1 protein.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MURF1 aa 2-14. The exact sequence is proprietary. (NP_115977)
    Sequence:

    DYKSSLIQDGNPM


    Database link: Q969Q1

  • Positive control
    • Human esophagus cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab183094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.

Target

  • Function
    E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • Tissue specificity
    Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • Developmental stage
    Expressed throughout all developmental stages.
  • Domain
    The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

Images

  • Anti-MURF1 antibody (ab183094) at 1/1050 dilution + lysate at 40 µg

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa


    Exposure time : 50 seconds

    10% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling MURF1 using ab183094 at a 1/60 dilution, Image on the right is treated with the synthetic peptide (original magnification: ×200).

References

ab183094 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 cells)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
30 µg
Treatment
0, 2, 5 mM Metformin for 24hrs
Specification
C2C12 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted Jan 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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