Overview

  • Product nameAnti-MURF1 antibody
    See all MURF1 primary antibodies
  • Description
    Rabbit polyclonal to MURF1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 1-14 (

    MDYKSSLIQDGNPM

    ) of Human MURF1 (NP_115977).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; 293T, A431, H1299, HeLaS3, Molt-4 and Raji cell lines

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionE3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • Tissue specificityMuscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • Developmental stageExpressed throughout all developmental stages.
  • DomainThe RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • Cellular localizationCytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

Anti-MURF1 antibody images

  • Anti-MURF1 antibody (ab96857) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
  • Immunofluorescence analysis of MURF1 in paraformaldehyde-fixed HeLa cells, using ab96857 at 1/200 dilution. Lower image is merged with DNA probe.

References for Anti-MURF1 antibody (ab96857)

This product has been referenced in:
  • MacNeil LG  et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . Read more (PubMed: 25289940) »
  • Bailey AN  et al. MAFbx, MuRF1, and the stress-activated protein kinases are upregulated in muscle cells during total knee arthroplasty. Am J Physiol Regul Integr Comp Physiol 303:R376-86 (2012). Human . Read more (PubMed: 22761181) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
Human Protein Reference Database shows that Murf1 may be expressed in liver tissue.
Please refer to:

http://www.hprd.org/summary?hprd_id=05843&isoform_id=05843_1&isoform_name=Isoform_1
Also,...

Read More
Application Western blot
Sample Pig Tissue lysate - other (Porcine skeletal muscle (longissimus dorsi))
Loading amount 30 µg
Specification Porcine skeletal muscle (longissimus dorsi)
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step StartingBlock (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
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Verified customer

Submitted Jan 19 2012

I am very pleased to hear you would like to accept our offer and test ab96857 in swine. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forswine and include this code in the &ldq...

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Thank you for your interest in Abcam's MuRF1 antibodies. I have performed a search of our catalog and specifically looked at ab77577 and ab96857 for suitability with swine. Unfortunately ab77577 is out of stock long term, however I agree that ab96857 w...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m)
Loading amount 5 µg
Specification Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m
Treatment LPS or NaCl as a control 48h
Gel Running Conditions Reduced Denaturing (4-12% bis-tris XT-Criterion BioRad)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"