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Goat polyclonal to MURF3
Synthetic peptide: GSAGPEEERPDGP, corresponding to C terminal amino acids 372-384 of Human MURF3.
Principal Names – RNF30; MURF; MURF-3; ring finger protein 30; muscle-specific RING-finger protein 3; muscle-specific RING-finger protein homolog.
Official Gene Symbol - RNF30.
GenBank Accession Number – NP_115935.
LocusLink ID - 57159 (human).
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. PubMed: 16477476
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with
Human MURF3 peptide (ab23024).
May bind and stabilize microtubules during myotubes formation.
Specifically expressed in heart and skeletal muscle.
Contains 1 B box-type zinc finger.
Contains 1 COS domain. Contains 1 RING-type zinc finger.
Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Associates with microtubules. Localizes to the Z-lines in skeletal muscles.
Information by UniProt
MURF 3 antibody
Western blot - MURF3 antibody (ab4351)
Predicted band size : 44 kDa ab4351 at a concentration of 1µg/ml staining MURF 3 in human muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour, detected by chemiluminescence.
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