Overview

  • Product name
  • Description
    Goat polyclonal to MURF3
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: GSAGPEEERPDGP, corresponding to C terminal amino acids 372-384 of Human MURF3.

  • General notes
    Principal Names – RNF30; MURF; MURF-3; ring finger protein 30; muscle-specific RING-finger protein 3; muscle-specific RING-finger protein homolog. Official Gene Symbol - RNF30. GenBank Accession Number – NP_115935. LocusLink ID - 57159 (human).

Properties

Applications

Our Abpromise guarantee covers the use of ab4351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml. PubMed: 16477476
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human MURF3 peptide (ab23024).

Target

  • Function
    May bind and stabilize microtubules during myotubes formation.
  • Tissue specificity
    Specifically expressed in heart and skeletal muscle.
  • Sequence similarities
    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Associates with microtubules. Localizes to the Z-lines in skeletal muscles.
  • Information by UniProt
  • Database links
  • Alternative names
    • MURF 3 antibody
    • MuRF antibody
    • MURF-3 antibody
    • MuRF3 antibody
    • Muscle specific RING finger protein 3 antibody
    • Muscle specific RING finger protein antibody
    • Muscle specific RING finger protein homolog antibody
    • Muscle-specific RING finger protein 3 antibody
    • Muscle-specific RING finger protein antibody
    • RING finger protein 30 antibody
    • RNF30 antibody
    • TRI54_HUMAN antibody
    • TRIM 54 antibody
    • Trim54 antibody
    • Tripartite motif containing 54 antibody
    • Tripartite motif containing protein 54 antibody
    • Tripartite motif-containing protein 54 antibody
    see all

Images



  • Predicted band size : 44 kDa


    ab4351 at a concentration of 1µg/ml staining MURF 3 in human muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour, detected by chemiluminescence.

References

This product has been referenced in:
  • Okamoto R  et al. FHL2 prevents cardiac hypertrophy in mice with cardiac-specific deletion of ROCK2. FASEB J 27:1439-49 (2013). Read more (PubMed: 23271052) »
  • Gregorio CC  et al. Functional properties of the titin/connectin-associated proteins, the muscle-specific RING finger proteins (MURFs), in striated muscle. J Muscle Res Cell Motil 26:389-400 (2005). Read more (PubMed: 16477476) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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