Overview

  • Product nameAnti-Mus81 antibody
    See all Mus81 primary antibodies
  • Description
    Rabbit polyclonal to Mus81
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 196 and 380 of Human Mus81 (NP_079404)

  • Positive control
    • HepG2, NIH-3T3 and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionInteracts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the XPF family.
    Contains 1 ERCC4 domain.
  • Developmental stageExpressed in S phase and G2 phase.
  • Cellular localizationNucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Anti-Mus81 antibody images

  • Anti-Mus81 antibody (ab97391) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Anti-Mus81 antibody (ab97391) at 1/500 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97391 at 1/100 dilution (Panel 1) and merged with DNA probe (Panel 2).

References for Anti-Mus81 antibody (ab97391)

ab97391 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Jul 14 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
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Submitted Jun 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 30 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Verified customer

Submitted Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"