Overview

  • Product name
  • Description
    Rabbit polyclonal to Mus81
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 196 and 380 of Human Mus81 (NP_079404)

  • Positive control
    • HepG2, NIH-3T3 and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • Developmental stage
    Expressed in S phase and G2 phase.
  • Cellular localization
    Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Anti-Mus81 antibody images

  • Anti-Mus81 antibody (ab97391) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Anti-Mus81 antibody (ab97391) at 1/500 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97391 at 1/100 dilution (Panel 1) and merged with DNA probe (Panel 2).

References for Anti-Mus81 antibody (ab97391)

ab97391 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Abcam user community

Verified customer

Submitted Jul 14 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
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Abcam user community

Verified customer

Submitted Jun 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
30 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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