The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23144634
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
Use at an assay dependent concentration.
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Belongs to the XPF family. Contains 1 ERCC4 domain.
Expressed in S phase and G2 phase.
Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunocytochemistry/ Immunofluorescence - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)This image is courtesy of an anonymous Abreview
ab14387 staining Mus81 in GM00637 Human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% FCS for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour 30 minutes at 37°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody (1/600).
Meisenberg C et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res45:1159-1176 (2017).
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