Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)

Overview

  • Product name
    Anti-Mus81 antibody [MTA30 2G10/3]
    See all Mus81 primary antibodies
  • Description
    Mouse monoclonal [MTA30 2G10/3] to Mus81
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Mus81 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab14387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23144634
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • Developmental stage
    Expressed in S phase and G2 phase.
  • Cellular localization
    Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Images

  • Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab14387 staining Mus81 in GM00637 Human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% FCS for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour 30 minutes at 37°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody (1/600).

    See Abreview

References

This product has been referenced in:

See all 23 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Treatment
cells were transfected with siMus81 siRNA
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 01 2017

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Remi Buisson

Verified customer

Submitted Oct 31 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
Username

Abcam user community

Verified customer

Submitted Jun 20 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
30 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Apr 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 p53wt cells)
Loading amount
30 µg
Specification
HCT116 p53wt cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 04 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (GM00637 human fibroblasts)
Specification
GM00637 human fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.4% Triton X-100
Blocking step
FCS as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Expression hMus81 in Baculovirus system)
Loading amount
10000 cells
Specification
Expression hMus81 in Baculovirus system
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Olga Mazin

Verified customer

Submitted Feb 12 2007

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up