Overview

  • Product name
    Anti-Musashi 1 / Msi1 antibody
    See all Musashi 1 / Msi1 primary antibodies
  • Description
    Rabbit polyclonal to Musashi 1 / Msi1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 176-362 of Human Musashi 1/ Msi1 (UniProt ID: O43347).

  • Positive control
    • 293T, H1299 and HepG2 cell lysates; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity
    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • Sequence similarities
    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The first RNA recognition motif binds more strongly to RNA compared to the second one.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Msi 1 antibody
    • Msi1 antibody
    • MSI1H_HUMAN antibody
    • Musashi homolog 1 antibody
    • Musashi RNA binding protein 1 antibody
    • Musashi-1 antibody
    • Musashi1 antibody
    • RNA binding protein Musashi homolog 1 antibody
    • RNA-binding protein Musashi homolog 1 antibody
    see all

Images

  • Anti-Musashi 1 / Msi1 antibody (ab154497) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling Musashi 1 / Msi1 using ab154497 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab154497 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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