Anti-Musashi 1 / Msi1 antibody - N-terminal (ab170378)

Overview

  • Product name
    Anti-Musashi 1 / Msi1 antibody - N-terminal
    See all Musashi 1 / Msi1 primary antibodies
  • Description
    Rabbit polyclonal to Musashi 1 / Msi1 - N-terminal
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Musashi 1/ Msi1 aa 74-101 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O43347

  • Positive control
    • CEM cell lysate; Human brain tissue; CEM cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.

Target

  • Function
    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity
    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • Sequence similarities
    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The first RNA recognition motif binds more strongly to RNA compared to the second one.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Msi 1 antibody
    • Msi1 antibody
    • MSI1H_HUMAN antibody
    • Musashi homolog 1 antibody
    • Musashi RNA binding protein 1 antibody
    • Musashi-1 antibody
    • Musashi1 antibody
    • RNA binding protein Musashi homolog 1 antibody
    • RNA-binding protein Musashi homolog 1 antibody
    see all

Images

  • Anti-Musashi 1 / Msi1 antibody - N-terminal (ab170378) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size : 39 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling Musashi 1 / Msi1 with ab170378 at a 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of CEM cells (right histogram) labeling Musashi 1 / Msi1 with ab170378 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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