Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178)

Overview

  • Product name
    Anti-muscarinic Acetylcholine Receptor 1 antibody
    See all Muscarinic Acetylcholine Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to muscarinic Acetylcholine Receptor 1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the internal region of human Muscarinic Acetylcholine Receptor 1

  • Positive control
    • Extracts from HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/5000.

Target

  • Relevance
    The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine.
  • Cellular localization
    Cell Membrane; multi-pass membrane protein
  • Database links
  • Alternative names
    • AChR antibody
    • Cholinergic receptor muscarinic 1 antibody
    • CHRM1 antibody
    • HM1 antibody
    • M1 antibody
    • Muscarinic acetylcholine receptor M1 antibody
    see all

Anti-muscarinic Acetylcholine Receptor 1 antibody images

  • All lanes : Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

References for Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178)

This product has been referenced in:
  • Falsafi SK  et al. Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems. PLoS One 7:e32082 (2012). WB ; Mouse . Read more (PubMed: 22384146) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Sample
Mouse Cell (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% Triton X 100 in PBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain tissue lysate, membrane fraction)
Loading amount
50 µg
Specification
brain tissue lysate, membrane fraction
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Apr 12 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

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