Anti-Muscarinic Acetylcholine Receptor 2 antibody (ab109050)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Muscarinic Acetylcholine Receptor 2 antibody
See all Muscarinic Acetylcholine Receptor 2 primary antibodies -
DescriptionRabbit polyclonal to Muscarinic Acetylcholine Receptor 2
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
Predicted to work with: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Muscarinic Acetylcholine Receptor 2.
-
Positive control
- This antibody gave a positive signal in Human Brain tissue lysate as well as SHSY5Y whole cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab109050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). Abcam recommends using milk as the blocking agent (3%). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
-
FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
-
Involvement in diseaseGenetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
-
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 522170 Cow
- Entrez Gene: 1129 Human
- Entrez Gene: 397498 Pig
- Omim: 118493 Human
- SwissProt: P41985 Cow
- SwissProt: P08172 Human
- SwissProt: P06199 Pig
- Unigene: 535891 Human
-
Alternative names
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
see all
Images
-
Western blot - Anti-Muscarinic Acetylcholine Receptor 2 antibody (ab109050)All lanes : Anti-Muscarinic Acetylcholine Receptor 2 antibody (ab109050) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
Additional bands at: 67 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Muscarinic Acetylcholine Receptor 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk as the blocking agent (3%).
References
ab109050 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"