Anti-Muscarinic Acetylcholine Receptor 2 antibody [B8E5] (ab90805)

Overview

  • Product name
    Anti-Muscarinic Acetylcholine Receptor 2 antibody [B8E5]
    See all Muscarinic Acetylcholine Receptor 2 primary antibodies
  • Description
    Mouse monoclonal [B8E5] to Muscarinic Acetylcholine Receptor 2
  • Tested applications
    Suitable for: WB, IHC-Fr, Functional Studies, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Cow, Pigeon, Pig, Chimpanzee, Pufferfish, Zebrafish, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to N-terminal part of the second extracellular loop, amino acids 168-192 (

    VRTVEDGECYIQFFSNAAVTFGTAI

    ) of Human Muscarinic Acetylcholine Receptor 2.

  • Epitope
    ab90805 recognizes the pentapeptide VRTVE (amino acids 168-172)
  • General notes


    ab90805 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody increases cGMP. ab90805 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and induces a small decrease in carbachol affinity for the M2 receptor.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purification notes
    0.2 µm filtered
  • Primary antibody notes
    ab90805 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody increases cGMP. ab90805 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and induces a small decrease in carbachol affinity for the M2 receptor.
  • Clonality
    Monoclonal
  • Clone number
    B8E5
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 52 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.
ICC/IF 1/50.

Target

  • Function
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
  • Involvement in disease
    Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7TM receptor antibody
    • Acetylcholine receptor, muscarinic, 2 antibody
    • AChR antibody
    • Acm2 antibody
    • ACM2_HUMAN antibody
    • Cholinergic receptor muscarinic 2 antibody
    • Cholinergic receptor, muscarinic 2, cardiac antibody
    • Cholinergic receptor, muscarinic 2, isoform a antibody
    • Cholinergic receptor, muscarinic 2a antibody
    • CHRM 2 antibody
    • CHRM2 antibody
    • chrm2a antibody
    • CM2 antibody
    • FLJ43243 antibody
    • HM 2 antibody
    • HM2 antibody
    • M2 antibody
    • M2 muscarinic receptor antibody
    • M2-mAChR antibody
    • MGC120006 antibody
    • MGC120007 antibody
    • Muscarinic acetylcholine receptor M2 antibody
    • Muscarinic M2 receptor antibody
    see all

References

This product has been referenced in:
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
  • Nascimento JH  et al. cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor. Am J Physiol Cell Physiol 281:C1251-8 (2001). Read more (PubMed: 11546662) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately we do not have blocking peptides for any of the antibody ab2805, ab90805 and ab109226. I apologize for the inconvenience this may have caused.

I can however recommend trying the following ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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