Recombinant
RabMAb

Anti-Muscarinic Acetylcholine Receptor 2 antibody [EPR4568] (ab109226)

Overview

  • Product name
    Anti-Muscarinic Acetylcholine Receptor 2 antibody [EPR4568]
    See all Muscarinic Acetylcholine Receptor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2
  • Specificity
    Please note: blast analysis of the immunogen sequence for ab109226 shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Muscarinic Acetylcholine Receptor 2 aa 150-250.
    Database link: P08172

  • Positive control
    • WB: SH-SY5Y, fetal brain, U87-MG, Neuro-2a, and Y79 lysates IHC-P: Human brain tissue IF: SH-SY5Y cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
    • Involvement in disease
      Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 7TM receptor antibody
      • Acetylcholine receptor, muscarinic, 2 antibody
      • AChR antibody
      • Acm2 antibody
      • ACM2_HUMAN antibody
      • Cholinergic receptor muscarinic 2 antibody
      • Cholinergic receptor, muscarinic 2, cardiac antibody
      • Cholinergic receptor, muscarinic 2, isoform a antibody
      • Cholinergic receptor, muscarinic 2a antibody
      • CHRM 2 antibody
      • CHRM2 antibody
      • chrm2a antibody
      • CM2 antibody
      • FLJ43243 antibody
      • HM 2 antibody
      • HM2 antibody
      • M2 antibody
      • M2 muscarinic receptor antibody
      • M2-mAChR antibody
      • MGC120006 antibody
      • MGC120007 antibody
      • Muscarinic acetylcholine receptor M2 antibody
      • Muscarinic M2 receptor antibody
      see all

    Anti-Muscarinic Acetylcholine Receptor 2 antibody [EPR4568] images

    • All lanes : Anti-Muscarinic Acetylcholine Receptor 2 antibody [EPR4568] (ab109226) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : U87-MG lysate
      Lane 4 : Neuro-2a lysate
      Lane 5 : Y79 lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100.
    • Immunofluorescent staining of SH-SY5Y cells using ab109226 at 1/100.

    References for Anti-Muscarinic Acetylcholine Receptor 2 antibody [EPR4568] (ab109226)

    This product has been referenced in:
    • O'Hern PJ  et al. Decreased microRNA levels lead to deleterious increases in neuronal M2 muscarinic receptors in Spinal Muscular Atrophy models. Elife 6:N/A (2017). WB ; Mouse . Read more (PubMed: 28463115) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Commercial Peroxidase blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
    Sample
    Human Tissue sections (Tendon)
    Specification
    Tendon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 14 2014

    Thank you very much for your email.

    As we are still retesting this antibody so customer data would be very helpful. Could you please ask customer few questions;

    - cell line used
    - Problem; high background, no staining etc.
    - a...

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    Thanks for the update.

    I will look forward to hearing back soon.

    I apologize for the delay.

    The product will be delivered tomorrow morning. The order number is 123772 (ab32087).

    I hope this information will be helpful.

    Thank you for contacting us.
    Your credit note ID is XXXXX.
    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purc...

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    I can send you the replacement, no problem!

    Could you confirm if you have received this antibody ab109226 as free of charge under the 100% Abreview testing promotion?

    Thanks!

    Thank you for your email and for providing the details.

    I have contacted our lab for the tubulin cross reactivity data. I will get back to you soon.

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I can confirm that, no other complaint was received for this antibody so the problem could be either with antibody or the protocol. We al...

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    Thank you for contacting us.

    Unfortunately we do not have blocking peptides for any of the antibody ab2805, ab90805 and ab109226. I apologize for the inconvenience this may have caused.

    I can however recommend trying the following ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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