Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab126168)

Overview

  • Product name
    Anti-Muscarinic Acetylcholine Receptor M3 antibody
    See all Muscarinic Acetylcholine Receptor M3 primary antibodies
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 286-461 of Human Muscarinic Acetylcholine Receptor M3 (UniProt P20309).

  • Positive control
    • NT2D1 whole cell lysate; Mouse brain lysate; U87-MG cell lysate IF/ICC: SKNSH cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab126168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab126168) at 1/1000 dilution + Mouse intestine tissue lysate at 14 µg

    Secondary
    HRP-conjugated goat anti-Rabbit IgG (H+L) polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 seconds

    This image is courtesy of an Abreview by Julien Chevalier.

    See Abreview

  • Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab126168) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size : 66 kDa
    7.5% SDS PAGE
  • Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab126168) at 1/1000 dilution + NT2D1 whole cell extracts at 30 µg

    Secondary
    HRP- conjugated anti-rabbit IgG

    Predicted band size : 66 kDa

    7.5% SDS PAGE

  • ICC/IF image of ab126168 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126158, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab126168 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

Submitted Sep 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

Submitted Jul 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Proximal colon)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
15 µg
Specification
Proximal colon
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2016

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Leider haben wir keinen anti Muscarinic Acetylcholine Receptor M3 Antikörper, welcher schon auf Mausgewebe und in der Immunfluorszenz (IF) oder in Immunoh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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