Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab41169)


  • Product nameAnti-Muscarinic Acetylcholine Receptor M3 antibody
    See all Muscarinic Acetylcholine Receptor M3 primary antibodies
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3
  • Specificityab41169 recognises the C terminal region of Muscarinic Acetylcholine Receptor M3. We have run several ELISA based assays and found no cross reactivity between this antibody and the M1, M2, M4 and M5 receptors.
  • Tested applicationsWB, ELISA, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide:


    (Rat) conjugated to KLH by a Cysteine residue linker

  • Positive control
    • PLP fixed rat brain sections, whole rat brain homogenate.



Our Abpromise guarantee covers the use of ab41169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 66 kDa. Block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. Dilute antibody in blocking buffer.With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/2500. PLP fixed.


  • FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AChR antibody
    • ACM3_HUMAN antibody
    • cholinergic receptor muscarinic 3 antibody
    • CHRM 3 antibody
    • CHRM3 antibody
    • EGBRS antibody
    • HM 3 antibody
    • HM3 antibody
    • m3 muscarinic acetylcholine receptor antibody
    • M3 muscarinic receptor antibody
    • muscarinic 3 antibody
    • Muscarinic acetylcholine receptor M3 antibody
    • muscarinic cholinergic m3 receptor antibody
    • muscarinic m3 receptor antibody
    see all

Anti-Muscarinic Acetylcholine Receptor M3 antibody images

  • ICC/IF image of ab41169 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41169) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab41169)

ab41169 has not yet been referenced specifically in any publications.

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Our record shows the antibody gave good signal even at 1/2500 dilution with PLP (periodate-lysine-paraformaldehyde fixative) fixed rat brain sections using ABC technique so optimization of dilution factor is highly re...

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Thank you for contacting us.

We apologize for the error; the datasheet has been updated by removing the concentration of the antibody. We recommend trying different dilutions e.g.1/100, 1/500 or 1/1000 for optimization.

I hope this in...

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I am very pleased to hear you would like to accept our offer and test ab41169 Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To re...

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Thank you very much for your interest in ab41169 Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end.

To our knowledge, ab41169 has not been tested in mouse.I performed a BLAST search, and itlooks like the peptide can...

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Leider haben wir keinen anti Muscarinic Acetylcholine Receptor M3 Antikörper, welcher schon auf Mausgewebe und in der Immunfluorszenz (IF) oder in Immunoh...

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I have received the following information from the laboratory which tested this antibody: " We have run several ELISA based assays and found no cross reactivity between this antibody and other non M3 receptors. Polypeptide ...

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