Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)

Overview

  • Product name
    Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2]
    See all Muscarinic Acetylcholine Receptor M4 primary antibodies
  • Description
    Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4
  • Specificity
    ab77956 reacts with Muscarinic acetylcholine receptor M4. There is no reactivity with the other subtypes.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Muscarinic Acetylcholine Receptor M4 i3 loop (human), fused to a tag.

  • Positive control
    • Transfected CHO cell lines
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab77956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
WB Use at an assay dependent dilution. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent dilution.

Target

  • Relevance
    The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • AChR antibody
    • Cholinergic receptor muscarinic 4 antibody
    • Chrm 4 antibody
    • CHRM4 antibody
    • HM 4 antibody
    • HM4 antibody
    • M4 antibody
    • Muscarinic acetylcholine receptor M4 antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
  • Zhao Z  et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci 10:71 (2016). IHC . Read more (PubMed: 27047336) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HGT101)
Loading amount
10 µg
Specification
HEK293, HGT101
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (STC1)
Loading amount
10 µg
Specification
STC1
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up