Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)

Overview

  • Product nameAnti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2]
    See all Muscarinic Acetylcholine Receptor M4 primary antibodies
  • Description
    Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4
  • Specificityab77956 reacts with Muscarinic acetylcholine receptor M4. There is no reactivity with the other subtypes.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Muscarinic Acetylcholine Receptor M4 i3 loop (human), fused to a tag.

  • Positive control
    • Transfected CHO cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab77956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
WB Use at an assay dependent dilution. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent dilution.

Target

  • RelevanceThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • AChR antibody
    • Cholinergic receptor muscarinic 4 antibody
    • Chrm 4 antibody
    • CHRM4 antibody
    • HM 4 antibody
    • HM4 antibody
    • M4 antibody
    • Muscarinic acetylcholine receptor M4 antibody
    see all

Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] images

  • Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)

This product has been referenced in:
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293, HGT101)
Loading amount 10 µg
Specification HEK293, HGT101
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (STC1)
Loading amount 10 µg
Specification STC1
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"