Anti-Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end (ab41170)

Overview

  • Product nameAnti-Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end
    See all Muscarinic Acetylcholine Receptor M4 primary antibodies
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M4 - Carboxyterminal end
  • SpecificityCross reactivity for ab41170: M4 Muscarinic Receptor (469-478): 100% M4 Muscarinic Receptor: 70% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M5 Muscarinic Receptor(519-531) M5 Muscarinic Receptor
  • Tested applicationsSuitable for: ICC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    peptide analogue of the C terminal of rat muscarinic M4 receptor covalently attached onto a carrier protein.

  • Positive control
    • Rat brain tissue, whole brain homogenate and cultured cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab41170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    ICC: 1/500.
    WB: 1/400. Predicted molecular weight: 53 kDa.
    This antibody is very sensitive to blocking agent. We recommend using whole goats serum for optimal results. Avoid any bovine products (BSA and milk).

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • AChR antibody
      • Cholinergic receptor muscarinic 4 antibody
      • Chrm 4 antibody
      • CHRM4 antibody
      • HM 4 antibody
      • HM4 antibody
      • M4 antibody
      • Muscarinic acetylcholine receptor M4 antibody
      see all

    References for Anti-Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end (ab41170)

    This product has been referenced in:
    • Falsafi SK  et al. Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems. PLoS One 7:e32082 (2012). WB ; Mouse . Read more (PubMed: 22384146) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"