Anti-Muscarinic Acetylcholine Receptor M5 antibody (ab41171)

Overview

  • Product nameAnti-Muscarinic Acetylcholine Receptor M5 antibody
    See all Muscarinic Acetylcholine Receptor M5 primary antibodies
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5 - Carboxyterminal end
  • SpecificityCross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor
  • Tested applicationsSuitable for: WB, ELISA, IHC (PFA fixed), IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide analogue of the C terminal of rat muscarinic M5 receptor covalently attached onto a carrier protein.

  • Positive control
    • Rat brain tissue, whole brain homogenate and cultured cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab41171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 60 kDa. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.
ELISA Use at an assay dependent concentration.
IHC (PFA fixed) 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23773170

Target

  • RelevanceThe muscarinic acetylcholine receptor M5 is a member of the GPCR family (subfamily, acetylcholine) which mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. The rimary transducing effect is PI turnover. The receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.
  • Cellular localizationMembrane; multi-pass membrane protein.
  • Database links
  • Alternative names
    • Acetylcholine receptor muscarinic 5 antibody
    • AChR antibody
    • AChR M5 antibody
    • CHKM5MR antibody
    • Cholinergic receptor muscarinic 5 antibody
    • Chrm 5 antibody
    • CHRM5 antibody
    • HM 5 antibody
    • HM5 antibody
    • m5 antibody
    • M5R antibody
    • MGC41838 antibody
    see all

References for Anti-Muscarinic Acetylcholine Receptor M5 antibody (ab41171)

This product has been referenced in:
  • Wasserman DI  et al. Cholinergic control of morphine-induced locomotion in rostromedial tegmental nucleus versus ventral tegmental area sites. Eur J Neurosci 38:2774-85 (2013). IHC-FoFr ; Mouse . Read more (PubMed: 23773170) »

See 1 Publication for this product

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I've looked through our records andto our knowledge, the antibody has only been tested inWestern blot, ELISA, and IHC on standard PFA-fixed tissue. I will get in touch with the lab and request i...

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