Recombinant Anti-ACTC1 antibody [EP184E] (ab46805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP184E] to ACTC1
- Suitable for: Flow Cyt (Intra), IHC-FoFr, IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ACTC1 antibody [EP184E]
See all ACTC1 primary antibodies -
Description
Rabbit monoclonal [EP184E] to ACTC1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-FoFr, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- A431 cell lysate; HeLa cells; human cardiac muscle
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP184E -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-FoFr |
Use at an assay dependent concentration.
|
|
IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/250 - 1/500.
|
|
WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
Notes |
---|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-FoFr
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
-
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:613424]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in ACTC1 are the cause of atrial septal defect type 5 (ASD5) [MIM:612794]. A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 533219 Cow
- Entrez Gene: 70 Human
- Entrez Gene: 11464 Mouse
- Entrez Gene: 29275 Rat
- Omim: 102540 Human
- SwissProt: P68032 Human
- SwissProt: P68033 Mouse
- SwissProt: P68035 Rat
see all -
Alternative names
- ACTC antibody
- ACTC_HUMAN antibody
- ACTC1 antibody
see all
Images
-
Anti-ACTC1 antibody [EP184E] (ab46805) at 1/1000 dilution + A431 cell lysate at 10ug/lane
Secondary
Goat anti-rabbit, HRP labelled. at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
-
Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab46805 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (21)
ab46805 has been referenced in 21 publications.
- Kra G et al. Effects of Environmental Heat Load on Endocannabinoid System Components in Adipose Tissue of High Yielding Dairy Cows. Animals (Basel) 12:N/A (2022). PubMed: 35327191
- Daddam JR et al. Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid. Int J Mol Sci 22:N/A (2021). PubMed: 33810070
- Das S et al. Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss. Commun Biol 3:330 (2020). PubMed: 32587337
- Zachut M et al. Seasonal heat load is more potent than the degree of body weight loss in dysregulating immune function by reducing white blood cell populations and increasing inflammation in Holstein dairy cows. J Dairy Sci 103:10809-10822 (2020). PubMed: 32896401
- Huang K et al. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer. Cell Metab 30:1107-1119.e8 (2019). PubMed: 31607564