Anti-muscle Actin antibody [EPR4791] (ab109499)


  • Product nameAnti-muscle Actin antibody [EPR4791]
    See all muscle Actin primary antibodies
  • Description
    Rabbit monoclonal [EPR4791] to muscle Actin
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human muscle Actin aa 200-300.

  • Positive control
    • Human skeletal muscle and Human heart lysates Human colonic tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similaritiesBelongs to the actin family.
    • Post-translational
      Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • ACTA2 antibody
      • ACTC antibody
      • ACTC1 antibody
      • Actin antibody
      • ACTS_HUMAN antibody
      • ACTSA antibody
      • Alpha 2 actin antibody
      • alpha skeletal muscle antibody
      • Alpha-actin-1 antibody
      • Cardiac muscle alpha actin 1 antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    Anti-muscle Actin antibody [EPR4791] images

    • All lanes : Anti-muscle Actin antibody [EPR4791] (ab109499) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Human heart lysates

      Lysates/proteins at 10 µg per lane.

      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
    • Immunohistochemical analysis of paraffin-embedded human colonic tissue using ab109499

    References for Anti-muscle Actin antibody [EPR4791] (ab109499)

    ab109499 has not yet been referenced specifically in any publications.

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