• Product nameAnti-muscle Actin antibody [HHF35]
    See all muscle Actin primary antibodies
  • Description
    Mouse monoclonal [HHF35] to muscle Actin
  • SpecificityThis antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers.
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The SDS extracted protein fraction of human myocardium.

  • General notes

    This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide
  • PurityTissue culture supernatant
  • Primary antibody notesThis antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.
  • ClonalityMonoclonal
  • Clone numberHHF35
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab7813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. PubMed: 24284066
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Prolonged fixation in buffered formalin can destroy the epitope.

IHC-Fr Use at an assay dependent concentration.


  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Anti-muscle Actin antibody [HHF35] images

  • Ab7813 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-muscle Actin antibody [HHF35] (ab7813)

This product has been referenced in:
  • Hoffmann C  et al. Live cell imaging reveals actin-cytoskeleton-induced self-association of the actin-bundling protein WLIM1. J Cell Sci 127:583-98 (2014). WB ; Human . Read more (PubMed: 24284066) »
  • Miettinen M Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. Am J Pathol 130:205-15 (1988). Read more (PubMed: 3276210) »

See all 5 Publications for this product

Product Wall

It has not been tested on mouse so we do not know how it will react, however as it was raised in mouse we believe the background would be too strong.