Anti-muscle Actin antibody [HHF35], prediluted (ab838)

Overview

  • Product name
    Anti-muscle Actin antibody [HHF35], prediluted
    See all muscle Actin primary antibodies
  • Description
    Mouse monoclonal [HHF35] to muscle Actin, prediluted
  • Specificity
    This antibody is specific for alpha and gamma specific actin isomers from skeletal, cardiac and smooth muscle but does not recognize beta and nonsmooth muscle gamma actin isomers. In Western blots, HHF35 picks up a 42 kDa protein from skeletal muscle but not from epithelial cell lines.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were injected with SDS extracted protein from human myocardium.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    HHF35
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
  • Target

    • Function
      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease
      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similarities
      Belongs to the actin family.
    • Post-translational
      modifications
      Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • ACTA2 antibody
      • ACTC antibody
      • ACTC1 antibody
      • Actin antibody
      • ACTS_HUMAN antibody
      • ACTSA antibody
      • Alpha 2 actin antibody
      • alpha skeletal muscle antibody
      • Alpha-actin-1 antibody
      • Cardiac muscle alpha actin 1 antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    Images

    • ab838 - immunohistochemistry

    References

    ab838 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I enquired with the antibody's originator but they were unable to provide any more information other than what is on the online datasheet. So I unfortunately can't tell you the dilution time.

    As I mentioned before, ab838 has been optimized for tissue staining. You're going to have to optimize your own dilutions to try using in Western blotting and I unfortunately can't make a recommendation.

    Thank you for your enquiry. Ab838 has been optimized for tissue section staining (it is prediluted)and is recommended for application in IHC. We also sell ab7813, Mouse monoclonal [HHF35] to muscle Actin, and although it has not yet been tested in West...

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    Thank you for your enquiry. Ab838 has been optimized for tissue section staining (it is prediluted)and is recommended for application in IHC. Other antibodies made from this clone, HHF35, have been used in Western blotting.

    Ab838 recognizes the alpha isomer.

    The originator of ab838 has informed me that they use various colors for coding these products. Green is for primary antibodies, yellow for link and red for label. It is just the food color added to the diluting buffer to differentiate various stainin...

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    All of our products (price, datasheet, publication, suitability, cross-reactivity) are listed in our on-line catalogue accessible at . I suggest that you should search for products that may interest you using the search box at the top of...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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