Recombinant
RabMAb

Anti-Muscleblind-like 1 antibody [EPR4565] (ab108519)

Overview

  • Product name
    Anti-Muscleblind-like 1 antibody [EPR4565]
    See all Muscleblind-like 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4565] to Muscleblind-like 1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Muscleblind-like 1.

  • Positive control
    • Jurkat, HeLa, and A673 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4565
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
    • Tissue specificity
      Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in disease
      Plays a role in the pathogenesis of dystrophia myotonica type 1 (DM1) [MIM:160900]. A muscular disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness and cardiac arrhythmias. Note=In muscle cells from DM1 patients, MBNL1 is sequestered by DMPK RNAs containing CUG triplet repeat expansions. MBNL1 binding is proportional to repeat length consistent with the direct correlation between the length of repeat expansion and disease severity.
    • Sequence similarities
      Belongs to the muscleblind family.
      Contains 4 C3H1-type zinc fingers.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasmic granule. Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress. Localized in the cytoplasm of multinucleated myotubes. Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy.
    • Information by UniProt
    • Database links
    • Alternative names
      • EXP antibody
      • EXP35 antibody
      • EXP40 antibody
      • EXP42 antibody
      • KIAA0428 antibody
      • MBNL antibody
      • MBNL protein antibody
      • MBNL1 antibody
      • MBNL1_HUMAN antibody
      • Muscleblind 41kD isoform antibody
      • Muscleblind like antibody
      • Muscleblind like protein 1 antibody
      • Muscleblind like splicing regulator 1 antibody
      • Muscleblind-like protein 1 antibody
      • Triplet expansion RNA binding protein antibody
      • Triplet-expansion RNA-binding protein antibody
      see all

    Images

    • All lanes : Anti-Muscleblind-like 1 antibody [EPR4565] (ab108519) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 42 kDa

    References

    This product has been referenced in:
    • Teplyuk NM  et al. Therapeutic potential of targeting microRNA-10b in established intracranial glioblastoma: first steps toward the clinic. EMBO Mol Med 8:268-87 (2016). WB ; Human . Read more (PubMed: 26881967) »
    • Alves S  et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins. Mol Neurodegener 11:58 (2016). IHC . Read more (PubMed: 27465358) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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