Anti-MUSK antibody (ab5510)

Overview

  • Product nameAnti-MUSK antibody
    See all MUSK primary antibodies
  • Description
    Rabbit polyclonal to MUSK
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 844-859 of Human MUSK.

  • Positive control
    • Placenta cell lysate.
  • General notes


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody notesProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 104 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionReceptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
  • Involvement in diseaseDefects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 FZ (frizzled) domain.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MDK 4 antibody
    • MDK4 antibody
    • MGC126323 antibody
    • MGC126324 antibody
    • Muscle antibody
    • Muscle skeletal receptor tyrosine kinase antibody
    • Muscle skeletal receptor tyrosine protein kinase antibody
    • Muscle specific kinase receptor antibody
    • Muscle specific tyrosine kinase receptor antibody
    • Muscle specific tyrosine protein kinase receptor antibody
    • Muscle-specific kinase receptor antibody
    • Muscle-specific tyrosine-protein kinase receptor antibody
    • MuSK antibody
    • MUSK_HUMAN antibody
    • Neural fold somite kinase 1 antibody
    • Neural fold somite kinase 2 antibody
    • Neural fold somite kinase 3 antibody
    • Neural fold somite kinase1 antibody
    • Neural fold somite kinase2 antibody
    • Neural fold somite kinase3 antibody
    • Nsk 1 antibody
    • Nsk 2 antibody
    • Nsk 3 antibody
    • Nsk1 antibody
    • Nsk2 antibody
    • Nsk3 antibody
    • Receptor tyrosine kinase MuSK antibody
    • Skeletal muscle receptor tyrosine kinase antibody
    • skeletal receptor tyrosine-protein kinase antibody
    see all

Anti-MUSK antibody images



  • Predicted band size : 104 kDa


    ab5510 at a 1/100 dilution staining approximately 97kDa of MUSK in placenta cell lysate by Western blot (ECL).
  • ab5510 staining MUSK in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-MUSK antibody (ab5510)

ab5510 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 mouse myotubes)
Loading amount 20 µg
Specification C2C12 mouse myotubes
Gel Running Conditions Reduced Denaturing (3-8% Tris-Acetate gel (Invitrogen))
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2013

Thank you for your enquiry. The originator of ab5510 screens their antibodies against a wide range of cell lines as part of the characterization of the antibody. For this antibody, placenta cell lysate tested positive. Regarding the band that you are s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"