Anti-MUSK antibody (ab92950)

Overview

  • Product nameAnti-MUSK antibody
    See all MUSK primary antibodies
  • Description
    Rabbit polyclonal to MUSK
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human MUSK.

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Skeletal Muscle Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab92950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

Target

  • FunctionReceptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
  • Involvement in diseaseDefects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 FZ (frizzled) domain.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MDK 4 antibody
    • MDK4 antibody
    • MGC126323 antibody
    • MGC126324 antibody
    • Muscle antibody
    • Muscle skeletal receptor tyrosine kinase antibody
    • Muscle skeletal receptor tyrosine protein kinase antibody
    • Muscle specific kinase receptor antibody
    • Muscle specific tyrosine kinase receptor antibody
    • Muscle specific tyrosine protein kinase receptor antibody
    • Muscle-specific kinase receptor antibody
    • Muscle-specific tyrosine-protein kinase receptor antibody
    • MuSK antibody
    • MUSK_HUMAN antibody
    • Neural fold somite kinase 1 antibody
    • Neural fold somite kinase 2 antibody
    • Neural fold somite kinase 3 antibody
    • Neural fold somite kinase1 antibody
    • Neural fold somite kinase2 antibody
    • Neural fold somite kinase3 antibody
    • Nsk 1 antibody
    • Nsk 2 antibody
    • Nsk 3 antibody
    • Nsk1 antibody
    • Nsk2 antibody
    • Nsk3 antibody
    • Receptor tyrosine kinase MuSK antibody
    • Skeletal muscle receptor tyrosine kinase antibody
    • skeletal receptor tyrosine-protein kinase antibody
    see all

Anti-MUSK antibody images

  • All lanes : Anti-MUSK antibody (ab92950) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-MUSK antibody (ab92950)

ab92950 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (HEK293 cells transfected with rat MuSK)
Loading amount 30 µg
Specification HEK293 cells transfected with rat MuSK
Gel Running Conditions Reduced Denaturing (7,5% polyacrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 06 2012

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Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participer à notre offre et tester ab92950 chez le rat (80% d'homologie entre l'immunogène et la protéine de rat, voir fic...

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