Recombinant Anti-Muted antibody [EPR11081(B)] (ab175205)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11081(B)] to Muted
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Muted antibody [EPR11081(B)]
See all Muted primary antibodies -
Description
Rabbit monoclonal [EPR11081(B)] to Muted -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, 293T and HeLa cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11081(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
IP
1/10 - 1/100. |
Target
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Function
The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. -
Sequence similarities
Belongs to the Muted family. - Information by UniProt
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Database links
- Entrez Gene: 63915 Human
- Entrez Gene: 17828 Mouse
- Entrez Gene: 306868 Rat
- Omim: 607289 Human
- SwissProt: Q8TDH9 Human
- SwissProt: Q8R015 Mouse
- SwissProt: B2GV52 Rat
- Unigene: 719272 Human
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Alternative names
- Biogenesis of lysosomal organelles complex 1, subunit 5, muted antibody
- Biogenesis of lysosome related organelles complex 1 subunit 5 antibody
- BLOC 1 subunit 5 antibody
see all
Images
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All lanes : Anti-Muted antibody [EPR11081(B)] (ab175205) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling Muted with ab175205 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175205 has not yet been referenced specifically in any publications.