Overview

  • Product nameAnti-MVP antibody [0.N.389]
    See all MVP primary antibodies
  • Description
    Mouse monoclonal [0.N.389] to MVP
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • Positive control
    • SKBR3 cells and breast carcinomas

Properties

Applications

Our Abpromise guarantee covers the use of ab14563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
Flow Cyt
IP
EMSA
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    GSA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT (staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
    IP: Use 2µg/mg protein lysate (Use Protein G).
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hrs at RT. Predicted molecular weight: 110 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
    • Tissue specificityPresent in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
    • Sequence similaritiesContains 9 MVP (vault) repeats.
    • DomainMVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.
    • Cellular localizationCytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    References for Anti-MVP antibody [0.N.389] (ab14563)

    ab14563 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab14563.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"