Overview

  • Product name
    Anti-MVP antibody [1032]
    See all MVP primary antibodies
  • Description
    Mouse monoclonal [1032] to MVP
  • Specificity
    The clone number has been updated from (0.N.389) to (1032) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    1032
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 104-110 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.
EMSA Use at an assay dependent concentration.

Target

  • Function
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificity
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similarities
    Contains 9 MVP (vault) repeats.
  • Domain
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localization
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

References for Anti-MVP antibody [1032] (ab14562)

This product has been referenced in:
  • Fowler PA  et al. In utero exposure to cigarette smoke dysregulates human fetal ovarian developmental signalling. Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24847019) »
  • Bellingham M  et al. Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. Mol Cell Endocrinol 376:156-72 (2013). IHC . Read more (PubMed: 23791816) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - other (monocytes)
Specification
monocytes
Blocking step
Roti-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

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Verified customer

Submitted Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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