Overview

  • Product name
    Anti-MVP antibody [1032]
    See all MVP primary antibodies
  • Description
    Mouse monoclonal [1032] to MVP
  • Specificity
    This antibody reacts with a 104 to 110 kD protein known as Major Vault Protein (MVP).
  • Tested applications
    Suitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

  • Positive control
    • Breast.
  • General notes

    MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.
  • Clonality
    Monoclonal
  • Clone number
    1032
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificity
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similarities
    Contains 9 MVP (vault) repeats.
  • Domain
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localization
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining MVP with ab2376.
  • ab2376 - immunohistochemistry

    Formalin fixed paraffin embedded human breast carcinoma stained with MVP, using ABC and DAB chromogen.

References

This product has been referenced in:
  • Durcin M  et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles 6:1305677 (2017). WB ; Mouse . Read more (PubMed: 28473884) »
  • Xu H  et al. Knock-down of ubiquitin-specific protease 22 by micro-RNA interference inhibits colorectal cancer growth. Int J Colorectal Dis : (2011). WB ; Human . Read more (PubMed: 21773699) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - other (Lung, endothelial cells)
Gel Running Conditions
Reduced Denaturing (7.5 %)
Loading amount
40 µg
Specification
Lung, endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Monica Giannotta

Verified customer

Submitted Jul 21 2015

I am very pleased to hear you would like to accept our offer and test ab2376 in Western blot on mouse. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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