Anti-MVP antibody [LRP-56] (ab3367)

Overview

  • Product nameAnti-MVP antibody [LRP-56]
    See all MVP primary antibodies
  • Description
    Mouse monoclonal [LRP-56] to MVP
  • Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    2R120 cell line derived by stepwise Doxorubicin exposure of the the SW-1573 non-small cell lung carcinoma cell line.

  • Positive control
    • Lung, Colon, kidney, bronchus or adrenal cortex (human tissue). Also in MDR cell lines such as GLC4/ADR, HT1080/DR4 & 2R120. Negative control antibody: Normal mouse serum

Properties

Applications

Our Abpromise guarantee covers the use of ab3367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC
IHC-Fr
IHC-P
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-Fr: Frozen tissues, cell lines, formalin-fixed/paraffin embedded tissues. 1/10 - 1/40. Pretreatment is not required. For optimal staining, the primary antibody should be incubated overnight at 4°C.
    IHC-P: 1/20.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
    • Tissue specificityPresent in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
    • Sequence similaritiesContains 9 MVP (vault) repeats.
    • DomainMVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.
    • Cellular localizationCytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    References for Anti-MVP antibody [LRP-56] (ab3367)

    ab3367 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your patience. I have received a reply from the originator of ab3367 (MVP antibody [LRP-56]), this antibody is an IgG2b kappa. Please don't hesitate to contact us again if you have further questions.

    Thank you for your enquiry; having looked into the problem it appears that this clone has been duplicated in our catalogue in error. I have removed the product added most recently to our catalogue and kept the long standing Ab3367.If you have any furth...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"