Overview

  • Product nameAnti-MX1 antibody
    See all MX1 primary antibodies
  • Description
    Rabbit polyclonal to MX1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Dog
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 246 of Human MX1.

  • Positive control
    • WB: H1299, HeLa, and Raji whole cell lysate; 293T, A431 and HepG2 cell lysates IHC-P: MDA-MB-468 xenograft

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by antigen affinity chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionMay regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus.
  • Sequence similaritiesBelongs to the dynamin family.
    Contains 1 GED domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human interferon regulated resistance GTP binding protein MXA antibody
    • IFI 78 antibody
    • IFI 78K antibody
    • IFI-78K antibody
    • IFI78 antibody
    • IFI78K antibody
    • Interferon induced GTP binding protein Mx1 antibody
    • Interferon induced protein p78 antibody
    • Interferon inducible protein p78 antibody
    • Interferon regulated resistance GTP binding protein antibody
    • Interferon regulated resistance GTP binding protein MxA antibody
    • Interferon-induced GTP-binding protein Mx1 antibody
    • Interferon-induced protein p78 antibody
    • Interferon-regulated resistance GTP-binding protein MxA antibody
    • MX 1 antibody
    • MX antibody
    • MX dynamin-like GTPase 1 antibody
    • MX1 antibody
    • MX1_HUMAN antibody
    • MxA antibody
    • Myxoma resistance protein 1 antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 antibody
    • Myxovirus (influenza) resistance 1 homolog of murine antibody
    • Myxovirus resistance 1, mouse, homolog of antibody
    • Myxovirus resistance protein 1 antibody
    • N-terminally processed antibody
    see all

Anti-MX1 antibody images

  • All lanes : Anti-MX1 antibody (ab95926) at 1/5000 dilution

    Lane 1 : H1299 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 76 kDa
  • Immunohistochemical analysis of paraffin embedded MDA-MB-468 xenograft, using ab95926 at a 1/500 dilution.

References for Anti-MX1 antibody (ab95926)

This product has been referenced in:
  • Norris EL  et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . Read more (PubMed: 27451424) »
  • Greenwood EJ  et al. Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections. PLoS Pathog 11:e1005146 (2015). IHC-P . Read more (PubMed: 26360709) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chimpanzee Tissue sections (Lymph Node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Lymph Node
Fixative Formaldehyde
Username

Dr. Edward Greenwood

Verified customer

Submitted Feb 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample African Green Monkey Tissue sections (Lymph Node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Lymph Node
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph Node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Lymph Node
Blocking step No blocking step used for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Thank you for contacting us.

To our knowledge, these products have not been tested for cross reactivity with Mx2. As the homology between the two protein is only 55%, we would not assume any cross reactivity however as this is not tested w...

Read More

Thank you for contacting us.
The recommended Hela cell lysates are positive control for WB.
For more information about IHC-P positive control, please click the following link;
http://www.proteinatlas.org/ENSG00000157601/cancer
I hope ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (inflamed spleen (monkey))
Specification inflamed spleen (monkey)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Blocking step Phosphate buffer containing hydrogen peroxide, 15 mmol/L NaN3 and detergent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"