Overview

  • Product name
  • Description
    Rabbit polyclonal to MX1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Dog
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 246 of Human MX1.

  • Positive control
    • WB: H1299, HeLa, and Raji whole cell lysate; 293T, A431 and HepG2 cell lysates IHC-P: MDA-MB-468 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by antigen affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus.
  • Sequence similarities
    Belongs to the dynamin family.
    Contains 1 GED domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human interferon regulated resistance GTP binding protein MXA antibody
    • IFI 78 antibody
    • IFI 78K antibody
    • IFI-78K antibody
    • IFI78 antibody
    • IFI78K antibody
    • Interferon induced GTP binding protein Mx1 antibody
    • Interferon induced protein p78 antibody
    • Interferon inducible protein p78 antibody
    • Interferon regulated resistance GTP binding protein antibody
    • Interferon regulated resistance GTP binding protein MxA antibody
    • Interferon-induced GTP-binding protein Mx1 antibody
    • Interferon-induced protein p78 antibody
    • Interferon-regulated resistance GTP-binding protein MxA antibody
    • MX 1 antibody
    • MX antibody
    • MX dynamin-like GTPase 1 antibody
    • MX1 antibody
    • MX1_HUMAN antibody
    • MxA antibody
    • Myxoma resistance protein 1 antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 antibody
    • Myxovirus (influenza) resistance 1 homolog of murine antibody
    • Myxovirus resistance 1, mouse, homolog of antibody
    • Myxovirus resistance protein 1 antibody
    • N-terminally processed antibody
    see all

Images

  • Lane 1 : Anti-MX1 antibody (ab95926) at 1/1000 dilution
    Lane 2 : Anti-MX1 antibody (ab95926)

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : Human MX-1 transfected 293T cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size : 76 kDa
  • Immunohistochemical analysis of paraffin embedded MDA-MB-468 xenograft, using ab95926 at a 1/500 dilution.
  • All lanes : Anti-MX1 antibody (ab95926) at 1/5000 dilution

    Lane 1 : H1299 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 76 kDa

References

This product has been referenced in:
  • Lee SJ  et al. Expression of Myxovirus Resistance A (MxA) Is Associated with Tumor-Infiltrating Lymphocytes in Human Epidermal Growth Factor Receptor 2 (HER2)-Positive Breast Cancers. Cancer Res Treat 49:313-321 (2017). IHC ; Human . Read more (PubMed: 27456948) »
  • Sullivan KD  et al. Trisomy 21 consistently activates the interferon response. Elife 5:N/A (2016). Read more (PubMed: 27472900) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chimpanzee Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Fixative
Formaldehyde
Username

Dr. Edward Greenwood

Verified customer

Submitted Feb 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
African Green Monkey Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Blocking step
No blocking step used for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Thank you for contacting us.

To our knowledge, these products have not been tested for cross reactivity with Mx2. As the homology between the two protein is only 55%, we would not assume any cross reactivity however as this is not tested w...

Read More

Thank you for contacting us.
The recommended Hela cell lysates are positive control for WB.
For more information about IHC-P positive control, please click the following link;
http://www.proteinatlas.org/ENSG00000157601/cancer
I hope ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (inflamed spleen (monkey))
Specification
inflamed spleen (monkey)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Blocking step
Phosphate buffer containing hydrogen peroxide, 15 mmol/L NaN3 and detergent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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