Overview

  • Product nameAnti-MXI1 antibody
    See all MXI1 primary antibodies
  • Description
    Rabbit polyclonal to MXI1
  • Specificityab29740 recognises MAX interacting protein 1 (MXI1)
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from residues 176 - 225 of human MXI1. Note: the amino acid sequence is proprietary).

  • Positive control
    • Fetal Brain lysate Human Lung tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX.
  • Tissue specificityHigh levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
  • Involvement in diseaseDefects in MXI1 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc11 antibody
    • Class C basic helix-loop-helix protein 11 antibody
    • MAD 2 antibody
    • MAD2 antibody
    • MAX dimerization protein 2 antibody
    • MAX interacting protein 1 antibody
    • Max interactor 1 antibody
    • Max related transcription factor antibody
    • MAX-interacting protein 1 antibody
    • MGC43220 antibody
    • MXD 2 antibody
    • MXD2 antibody
    • MXI 1 antibody
    • MXI antibody
    • mxi1 antibody
    • MXI1 protein antibody
    • MXI1_HUMAN antibody
    • Protein MXI1 antibody
    see all

Anti-MXI1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling MXI1 with ab28740 at 4-8µg/ml. Positively labelled cells are (indicated by arrows) are bronchiole epithelial cells. Magnification: X400.

  • Anti-MXI1 antibody (ab28740) at 1 µg/ml + Foetal brain lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000 - 1/100,000

    Predicted band size : 33 kDa
    Observed band size : ~37 kDa (why is the actual band size different from the predicted?)

References for Anti-MXI1 antibody (ab28740)

ab28740 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"