Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab93835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 149 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMay activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity).
  • Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on XPO1/CRM1 (By similarity). Predominantly nucleolar.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024407 antibody
    • AU019902 antibody
    • cb486 antibody
    • FLJ37886 antibody
    • MBB1A_HUMAN antibody
    • MYB binding protein (P160) 1a antibody
    • myb binding protein (P160) 1a like antibody
    • Myb-binding protein 1A antibody
    • Mybbp1a antibody
    • nuclear protein P160 antibody
    • P160 antibody
    • p160MBP antibody
    • p53 activated protein 2 antibody
    • p67MBP antibody
    • PAP2 antibody
    • PAR interacting protein antibody
    • RP23 48A2.3 antibody
    • sb:cb486 antibody
    see all

Anti-MYBBP1A antibody images

  • All lanes : Anti-MYBBP1A antibody (ab93835) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 149 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab93835 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93835, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-MYBBP1A antibody (ab93835)

ab93835 has not yet been referenced specifically in any publications.

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