Overview

  • Product name
  • Description
    Rabbit polyclonal to MYBBP1A
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Immunogen corresponds to a region within amino acids 450-500 of Human MYBBP1A (NP_055335.1).

  • Positive control
    • HeLa whole cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab99361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity).
    • Cellular localization
      Cytoplasm. Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on XPO1/CRM1 (By similarity). Predominantly nucleolar.
    • Information by UniProt
    • Database links
    • Alternative names
      • AL024407 antibody
      • AU019902 antibody
      • cb486 antibody
      • FLJ37886 antibody
      • MBB1A_HUMAN antibody
      • MYB binding protein (P160) 1a antibody
      • myb binding protein (P160) 1a like antibody
      • Myb-binding protein 1A antibody
      • Mybbp1a antibody
      • nuclear protein P160 antibody
      • P160 antibody
      • p160MBP antibody
      • p53 activated protein 2 antibody
      • p67MBP antibody
      • PAP2 antibody
      • PAR interacting protein antibody
      • RP23 48A2.3 antibody
      • sb:cb486 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MYBBP1A with ab99361 at 1/1000 (1µg/ml). Detection: DAB.
    • ICC/IF image of ab99361 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99361 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) HepG2, HEk293, and MCF-7 cells at 1ug/ml.

    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99361 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

      Western Blot: rabbit anti-MYBBP1A antibody at 1 µg/ml

      developed using the ECL technique

      Exposure time : 1 second

      Predicted band size : 149 kDa

    References

    ab99361 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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