Overview

  • Product name
    Anti-Myc tag antibody [9E10] (DyLight® 650)
    See all Myc tag primary antibodies
  • Description
    Mouse monoclonal [9E10] to Myc tag (DyLight® 650)
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm
  • Specificity
    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    , corresponding to amino acids 410-419 of Human c-Myc.

Properties

Applications

Our Abpromise guarantee covers the use of ab117487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

References

This product has been referenced in:
  • McConnell AD  et al. High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation. PLoS One 7:e49458 (2012). Flow Cyt ; Human . Read more (PubMed: 23166676) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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