Anti-Myc tag antibody [9E10] (HRP) (ab62928)

Overview

  • Product name
    Anti-Myc tag antibody [9E10] (HRP)
    See all Myc tag primary antibodies
  • Description
    Mouse monoclonal [9E10] to Myc tag (HRP)
  • Conjugation
    HRP
  • Specificity
    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Synthetic peptide corresponding to Human c-Myc aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/500.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Images

  • Anti-Myc tag antibody [9E10] (HRP) (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References

This product has been referenced in:
  • Huang J  et al. Linc-RoR promotes c-Myc expression through hnRNP I and AUF1. Nucleic Acids Res 44:3059-69 (2016). RT-PCR . Read more (PubMed: 26656491) »
  • Shao W  et al. FBXO3 Protein Promotes Ubiquitylation and Transcriptional Activity of AIRE (Autoimmune Regulator). J Biol Chem 291:17953-63 (2016). Read more (PubMed: 27365398) »

See all 15 Publications for this product

Customer reviews and Q&As

The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller q...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20 µg
Specification
293T cells
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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