HRP Anti-Myc tag antibody [9E10] (ab62928)
Key features and details
- HRP Mouse monoclonal [9E10] to Myc tag
- Suitable for: ELISA, WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
HRP Mouse monoclonal [9E10] to Myc tag -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
contains 0.4M Arginine -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
9E10 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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Assay kits
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/100 - 1/500.
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).
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Notes |
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ELISA
1/100 - 1/500. |
WB
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa). |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
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Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution +
Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesBlocking buffer: 2% BSA
Gel type: MOPS -
HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab62928 has been referenced in 30 publications.
- Daoud M et al. XIAP promotes melanoma growth by inducing tumour neutrophil infiltration. EMBO Rep 23:e53608 (2022). PubMed: 35437868
- He W et al. Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells. Oncogene 41:4537-4546 (2022). PubMed: 36064578
- Jin JO et al. Sequential ubiquitination of p53 by TRIM28, RLIM, and MDM2 in lung tumorigenesis. Cell Death Differ 28:1790-1803 (2021). PubMed: 33328571
- Shailesh H et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
- Cho JH et al. Engineering advanced logic and distributed computing in human CAR immune cells. Nat Commun 12:792 (2021). PubMed: 33542232