Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLink® (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-sterilized (0.2 µm).
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19679075
WB 1/500. Can be blocked with Human c-Myc peptide (ab13837).
IP 1/250.
ICC 1/250.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

References

This product has been referenced in:
  • Rutkowska-Wlodarczyk I  et al. A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins. J Neurosci 35:5171-9 (2015). Read more (PubMed: 25834043) »
  • Selak S  et al. A role for SNAP25 in internalization of kainate receptors and synaptic plasticity. Neuron 63:357-71 (2009). ICC/IF . Read more (PubMed: 19679075) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 16 2014

A replacement vial is being sent to you and you should receive it tomorrow. For your record it is order# 39089. I did receive some information from the antibody's originator which I hope will also help you out concerning cell fixation. "We have fou...

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Thank you for your patience. I was away on vacation and could not get back to you earlier. We have had no other complaints regarding this antibody. You're getting absolutely no signal? Are you doing antigen retrieval? You stated that your cells are ...

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I looked at the tracking for Fed Ex and it states that the package was delivered on Feb 25 at 11am and signed for by L. Kim. Although the antibody arrived a day late, it should not affect the antibody (it should have still been cold). If you continue t...

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We don't have an unlabeled goat anti-chicken IgY antibody (all of ours are conjugated). Aves lab has one: Cat.# U-1010. If you have any more questions, please contact us again.

I contacted the supplier of ab172, and they provided me with the following information. Chicken IgY's lack an Fc domain in the "nu" heavy chain which where proteins A and G normally bind. Consequently, protein A/G-Agarose cannot be used directly t...

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I'm afraid that I don't know of anyone who has used the c-myc antibodies for immunofluorescence in yeast, either. Sorry.

Dear Kathleen, has Aves replied to this yet? If not their direct email is: info@aveslab.com

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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