Overview

  • Product nameAnti-Myc tag antibody
    See all Myc tag primary antibodies
  • Description
    Rabbit polyclonal to Myc tag
  • SpecificityELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
  • Tested applicationsSuitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt, ICC/IF, ICC, Electron Microscopymore details
  • Immunogen

    EQKLISEEDL

    (C-MYC) conjugated with KLH.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19793804

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 16093346
ICC Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).

Target

  • RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localizationNuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-Myc tag antibody images

  • ab9106 staining the Myc tag in HeLa cells transfected with a Myc-tagged membrane targeting protein by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab9106 at 4µg/mg lysate used in human huh-7 whole cell lysate (1x106 cells). Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution ab17437.

    See Abreview

  • Ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate). In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.

    Lane 1: Input untransfected cells

    Lane 2: Immunoprecipitated untransfected cells

    Lane 3: Input co-transfected cells

    Lane 4: Immunoprecipitated co-transfected cells

    This image is courtesy of an Abreview submitted on 23 March 2006.

    See Abreview

  • ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC. The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10nm Gold conjugated) was used as the secondary. The image shown is immuno electron microscopical staining with thawed cryosections.

    See Abreview

References for Anti-Myc tag antibody (ab9106)

This product has been referenced in:
  • Kim DI  et al. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell 27:1188-96 (2016). ICC/IF . Read more (PubMed: 26912792) »
  • Qi Y  et al. A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. Nat Commun 7:ncomms11840 (2016). WB . Read more (PubMed: 27291620) »

See all 121 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293 Cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment Transfected with Myc-GFP plasmid.
Specification 293 Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment lipofectamine 2000 transfected
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 0.3% BSA/8%donkeyserum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Karen Yee

Verified customer

Submitted Oct 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat, K562, and HL-60 cells)
Loading amount 50000 cells
Specification Jurkat, K562, and HL-60 cells
Gel Running Conditions Reduced Denaturing (4-20% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Tobacco Tissue lysate - whole (leaf)
Loading amount 20 µg
Specification leaf
Gel Running Conditions Non-reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Thank you for your email.

Unfortunately the delay wasn't from Abcam, we have shipped the product at correct temperature. Please check your delivery service and let them know these products are temperature sensitive and they need to be delivere...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 25 µg
Specification 293T
Treatment Cells transfected with K8-Myc-tag
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 03 2012

Thank you for contacting us.

From the looks of it, this is not a suitable antibody for you, as it is only suitable for the tag (only a few AA long)but not for the c-Myc protein (48 kDa). I checked the sequence and it looks like this antibody...

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DISCOUNT CODE: ***
Expiration date: June 17th, 2012
I am very pleased to hear you would like to accept our offer and test ab9106 in ChIP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please ...

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