Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgY
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/200 - 1/500 (coating) and 1/5000 – 1/20000 (probing).
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localizationNuclear
    • Alternative names
      • avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c-Myc antibody
      • class E basic helix-loop-helix protein 39 antibody
      • MRTL antibody
      • MYC antibody
      • Myc Epitope Tag antibody
      • myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • oncogene c-Myc antibody
      • proto-oncogene c-Myc antibody
      • protooncogene homologous to myelocytomatosis virus antibody
      • transcription factor p64 antibody
      • v-myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      see all

    References for Anti-Myc tag antibody (ab9109)

    This product has been referenced in:
    • Wang F  et al. Ca2+ homeostasis modulation enhances the amenability of L444P glucosylcerebrosidase to proteostasis regulation in patient-derived fibroblasts. ACS Chem Biol 6:158-68 (2011). ELISA . Read more (PubMed: 21043486) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for contacting us with your question.

    Since the antibody ab9109 is specific for the Myc tag and not an endogenousDrosophila protein, we would guarantee this antibody to work in the tested applications of Western blot and E...

    Read More

    We do not have any data on the use of this antibody in immunofluorescence. However, if you are testing the antibody in this application, please let us know your results and in return we'll forward a EUR 25.00 Amazon gift voucher.

    I'm sorry, I don't quite understand your question. In WB, this antibody is reported to be able to detect the c-myc epitope tag at a 1:10,000 dilution.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"