Overview

  • Product nameAnti-Myc tag antibody (Agarose)
    See all Myc tag primary antibodies
  • Description
    Goat polyclonal to Myc tag (Agarose)
  • ConjugationAgarose
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: ChIP
  • Immunogen

    Full length native protein

    EQKLISEEDL

    (c-myc) (purified): conjugated to KLH.

  • General notes

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab1253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.
  • Application notesIs unsuitable for ChIP.
  • Target

    • RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localizationNuclear
    • Alternative names
      • avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c-Myc antibody
      • class E basic helix-loop-helix protein 39 antibody
      • MRTL antibody
      • MYC antibody
      • Myc Epitope Tag antibody
      • myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • oncogene c-Myc antibody
      • proto-oncogene c-Myc antibody
      • protooncogene homologous to myelocytomatosis virus antibody
      • transcription factor p64 antibody
      • v-myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      see all

    References for Anti-Myc tag antibody (Agarose) (ab1253)

    This product has been referenced in:
    • Carballo JA  et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet 9:e1003545 (2013). IP . Read more (PubMed: 23825959) »
    • Warrington SJ  et al. The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2. Development 140:1045-54 (2013). IP . Read more (PubMed: 23364328) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK 293T)
    Total protein in input 25 µg
    Specification HEK 293T
    Immuno-precipitation step Other - Pre-bound agarose
    Username

    Dr. Jonathan Rud

    Verified customer

    Submitted Dec 28 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Immuno-precipitation step Other
    Username

    Abcam user community

    Verified customer

    Submitted Nov 03 2005

    Thank you for your enquiry. You don't need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this anti...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"