Overview

  • Product name
    Anti-Myc tag antibody (Biotin)
    See all Myc tag primary antibodies
  • Description
    Rabbit polyclonal to Myc tag (Biotin)
  • Conjugation
    Biotin
  • Specificity
    This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxytermini of targeted proteins in transfected or transformed cells.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Immunogen

    Synthetic peptide corresponding to Myc tag aa 410-419 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    EQKLISEEDL

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB Use a concentration of 0.5 - 1 µg/ml.
ICC/IF 1/400.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Images

  • This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescence analysis of B16 melanoma cells transfected with a fusion protein with Myc tag, staining Myc tag with ab34773.

    Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocked with 0.2% BSA for 16 hours at 4°C. Samples were incubated with primary antibody (1/400 in PBS/ BSA 0.2%/ saponin 0.05%) for 1 hour. AlexaFluor®488-conjugated streptavidin (1/500) was used to detect staining

    See Abreview

References

This product has been referenced in:
  • Hayes HL  et al. Pdx-1 activates islet a- and ß-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol 33:4017-29 (2013). Read more (PubMed: 23938296) »

See 1 Publication for this product

Customer reviews and Q&As

Merci de nous avoir contactés.

Ce code vous donnera un avoir de €380.00 sur votre prochaine commande.

Les frais de ports seront pris en charge pour ce produit uniquement. C'est-à-dire, si vous passer une commande ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16)
Specification
B16
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS/ BSA 0.2%/ SAPONIN 0.05%
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 4°C
Username

Ms. Alice Rochard

Verified customer

Submitted Dec 03 2012

Merci de nous avoir contactés.

Oui tout à fait. Vous pouvez utilisez ce code pour l’achat d’un autre anticorps différent que celui déjà testé pour lequel vous nous avez soumis vos ré...

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Suite à notre conversation téléphonique, je vous envoie les codes pour l’abtrial. Comme je l'ai expliqué au téléphone, normalement nous donnons ce code qu’avant l'achat du produit cependant, je suis...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells over-expressing pGEN IRES pyk2 myc t)
Loading amount
10 µg
Specification
NIH 3T3 cells over-expressing pGEN IRES pyk2 myc t
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 over-expressing pGEN IRES Mer myc tag)
Loading amount
10 µg
Specification
HEK 293 over-expressing pGEN IRES Mer myc tag
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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