Overview

  • Product nameAnti-Myc tag antibody - ChIP Grade
    See all Myc tag primary antibodies
  • Description
    Goat polyclonal to Myc tag - ChIP Grade
  • SpecificityAntibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
  • Tested applicationsSuitable for: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    (c-myc) conjugated to KLH.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-4 µg/mg of lysate.

Also PubMed: 20224777 

 

ChIP Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt Use at an assay dependent concentration. PubMed: 22158898

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/200 - 1/2000.
ELISA 1/100 - 1/500.

Target

  • RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localizationNuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-Myc tag antibody - ChIP Grade images

  • All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg
  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary.

    See Abreview

  • ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.  

References for Anti-Myc tag antibody - ChIP Grade (ab9132)

This product has been referenced in:
  • Weider M  et al. Elevated in vivo levels of a single transcription factor directly convert satellite glia into oligodendrocyte-like cells. PLoS Genet 11:e1005008 (2015). Read more (PubMed: 25680202) »
  • Takasawa K  et al. FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice. FASEB J 28:2020-8 (2014). ChIP . Read more (PubMed: 24451388) »

See all 27 Publications for this product

Product Wall

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Thank you very much for your call today and for your question about ab9132 and our other myc tag antibodies.

I couldn't find any data specifically showing that ab9132 has been tested for reactivity against endogenous c-myc, however since the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - other (HEK cell Chromatin)
Specification HEK cell Chromatin
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Real-time PCR
Positive control Histone H3 antibody
Negative control IGG antibody, EV Control
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Verified customer

Submitted Oct 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cell)
Loading amount 20 µg
Specification 293 cell
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Permeabilization Yes - 0.15% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Aug 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (BY4741 strain)
Loading amount 100000 cells
Specification BY4741 strain
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 23 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (skeleton)
Specification skeleton
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 17 2007

Thank you for your enquiry. Unfortunately the antibody has not been tested on endogenous mouse protein, so there is no data to say either way. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything furt...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (Jurkat cell lines)
Specification Jurkat cell lines
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control A stable Jurkat cell line expressing a myc- tagged version of rtTa-MYC.HBP1
Primers flanking the binding site of HBP1 on hCD2-LCR were used as a positive control
Negative control As a negative control a Jurkat cell line that does not express the myc-tagged protein: rtTA was employed.
The HPRT gene was used as a negative control locus
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Submitted Nov 07 2006

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"