Our Abpromise guarantee covers the use of ab9132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 1-4 µg/mg of lysate.
Also PubMed: 20224777
|ChIP||Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22158898
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC||1/200 - 1/2000.|
|ELISA||1/100 - 1/500.|
ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.