Overview

  • Product name
    Anti-Myc tag antibody (FITC)
    See all Myc tag primary antibodies
  • Description
    Rabbit polyclonal to Myc tag (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Immunogen

    EQKLISEEDL (C-MYC) conjugated with KLH.

  • General notes

    Molar F/P ratio is 4.6:1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250. PubMed: 17443800
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ELISA 1/1000.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

References

This product has been referenced in:
  • Xiao C  et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). Read more (PubMed: 17443800) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your interest in ab1393.

Unfortunately we do not have more information about the use of the anti-Myc tag antibody (FITC) ab1393 in ICC/IF than the one available in the publication (PubMed: 17443800) and we do not curren...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up