Anti-Myc tag antibody (FITC) (ab1394)

Overview

  • Product name
    Anti-Myc tag antibody (FITC)
    See all Myc tag primary antibodies
  • Description
    Chicken polyclonal to Myc tag (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Immunogen

    EQKLISEEDL ( C-MYC) conjugated with KLH.

  • General notes

    Molar F/P ratio is 5.6:1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Light chain type
    unknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt 1/100.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

References

ab1394 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Yeast expressing myc-tagged peptide)
Specification
Yeast expressing myc-tagged peptide
Fixation
none
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted May 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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