Overview

  • Product name
    Anti-Myc tag antibody [Myc.A7]
    See all Myc tag primary antibodies
  • Description
    Mouse monoclonal [Myc.A7] to Myc tag
  • Specificity
    Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Tested applications
    Suitable for: ICC/IF, ELISA, Dot blot, WB, IPmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    , corresponding to amino acids 410-419 of Human Myc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Protein A affinity chromatography
  • Clonality
    Monoclonal
  • Clone number
    Myc.A7
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB 1/1000. Can be blocked with Human c-Myc peptide (ab13837).
IP Use at an assay dependent concentration.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-Myc tag antibody [Myc.A7] images

  • All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution

    Lane 1 : HEK293 lysate - untransfected
    Lane 2 : HEK293 lysate - transfected with Myc-tagged protein
  • Top Panel: ab18185 staining Myc Tag in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody.
    Bottom panel: Synapses (Red); Anti-myc tag (Green)

    See Abreview

References for Anti-Myc tag antibody [Myc.A7] (ab18185)

This product has been referenced in:
  • Yu Z  et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. Elife 5:N/A (2016). Read more (PubMed: 27063938) »
  • Tang J  et al. Parkin Protects against Oxygen-Glucose Deprivation/Reperfusion Insult by Promoting Drp1 Degradation. Oxid Med Cell Longev 2016:8474303 (2016). WB . Read more (PubMed: 27597885) »

See all 7 Publications for this product

Product Wall

Thank you for your enquiry,

I am sorry to confirm that the Kd for ab18185 Myc tag antibody [Myc.A7] has not been assessed. It is unusual for the Kd to be measured for research grade antibodies.

We aim to provide as much informatio...

Read More

Thank you for getting back to me and for providing some further information about your research project.

Currently, we have 2 monoclonal antibodies against Myc tag: ab18185 and ab94936. Although they are from the same clone but from two sourc...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
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Verified customer

Submitted Mar 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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