The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 5 µg/ml.
FunctionMay control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
Tissue specificityHighly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
Sequence similaritiesBelongs to the AMY1 family.
Cellular localizationCytoplasm. Nucleus. Mitochondrion. Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
ICC/IF image of ab66331 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66331 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - MYCBP antibody (ab66331)
Anti-MYCBP antibody (ab66331) at 0.3 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 12 kDa Observed band size : 12 kDa