Overview

  • Product name
  • Description
    Rabbit polyclonal to MYCBP
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: TPENPEIELL RLELAEMKEK YEAIVEENKK LKAKLAQYEP PQEEKRAE, corresponding to amino acids 56-103 of Human MYCBP

  • Positive control
    • Jurkat cell lysate and human kidney tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: JEG3.

Properties

Applications

Our Abpromise guarantee covers the use of ab66331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
  • Tissue specificity
    Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
  • Sequence similarities
    Belongs to the AMY1 family.
  • Cellular localization
    Cytoplasm. Nucleus. Mitochondrion. Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMY 1 antibody
    • AMY-1 antibody
    • AMY1 antibody
    • Associate of Myc 1 antibody
    • C myc binding protein antibody
    • C-Myc-binding protein antibody
    • Cmyc binding protein antibody
    • MYCBP antibody
    • MYCBP_HUMAN antibody
    see all

Images

  • ICC/IF image of ab66331 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66331 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MYCBP antibody (ab66331) at 0.3 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ab66331 at 4µg/ml staining MYCBP in epithelial cells of human kidney renal tubule (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.
  • Immunohistochemistry of Human Intestine tissue at an antibody concentration of 4-8µg/ml using anti-MYCBP antibody (ab66331)

References

ab66331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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