Anti-Mycobacterium tuberculosis 16kDa antibody [HTM63] (ab22746)

Overview

  • Product nameAnti-Mycobacterium tuberculosis 16kDa antibody [HTM63]
    See all Mycobacterium tuberculosis 16kDa primary antibodies
  • Description
    Mouse monoclonal [HTM63] to Mycobacterium tuberculosis 16kDa
  • Specificityab22746 recognises native and recombinant M. tuberculosis 16 kDa antigen.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Recombinant full length protein: M. tuberculosis 16 kDa antigen.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide.
    Constituents: PBS, pH 7.2.
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab22746 has been purified from ascites. It is >95% pure and its purity has been tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberHTM63
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
    • Cellular localizationSecreted
    • Alternative names
      • 16 kDa antigen antibody
      • 16 kDa immunoprotective extracellular protein antibody
      • Antigen MPT63 antibody
      • MT1977 antibody
      • MTCY09F9.38 antibody
      • Rv1926c antibody
      see all

    References for Anti-Mycobacterium tuberculosis 16kDa antibody [HTM63] (ab22746)

    ab22746 has not yet been referenced specifically in any publications.

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    The Rv1926c sequence was the immunogen sequence used for development of this clone. AB22746 will recognize an epitope within the Rv1926c sequence, but the exact epitope location within this sequence has not bee...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"