Anti-Mycobacterium tuberculosis Ag85 antibody (ab36731)


  • Product nameAnti-Mycobacterium tuberculosis Ag85 antibody
  • Description
    Mouse monoclonal to Mycobacterium tuberculosis Ag85
  • SpecificityThis antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
  • Immunogen

    Bacterial press extract from Mycobacterium tuberculosis H37Rv

  • EpitopeEpitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
  • General notes

    WHO no. IT-49


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab36731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
WB Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.


  • RelevanceMycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
  • Alternative names
    • Antigen 85 complex A antibody
    • Antigen 85 complex B antibody
    • Antigen 85 complex C antibody
    • fbpA antibody
    • fbpB antibody
    • fbpC antibody
    • Secreted antigen 85 A antibody
    • Secreted antigen 85 B antibody
    • Secreted antigen 85 C antibody
    see all

References for Anti-Mycobacterium tuberculosis Ag85 antibody (ab36731)

This product has been referenced in:
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »
  • Rengarajan J  et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . Read more (PubMed: 18172199) »

See all 4 Publications for this product

Product Wall

HYT 27, does react in WB with both Ag85A, B and C, see the paper by Armitige LY and colleagues, whom used HYT 27 in WB (on page 774, fbpA antibody) and showed very good reaction with A.

Armitage et al., INFECTION AND IMM...

Read More

Thank you for contacting us.

I have had the opportunity to speak with the lab regarding the specificity and cross reactivity of ab36731.


HYT 27 is specific for Mycobacterium mycolyltransferase. ...

Read More

Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (transformed E coli)
Loading amount 0.01 µg
Specification transformed E coli
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 29 2007