Mouse monoclonal to Mycobacterium tuberculosis Ag85
This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein.
No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.