Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019)

Overview

  • Product nameAnti-Mycobacterium tuberculosis Ag85B antibody
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis Ag85B
  • SpecificityThis antibody is specific for the Ag85B protein in sera, plasma or cell culture supernatant.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with Mycobacterium tuberculosis. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein (Mycobacterium tuberculosis)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.05% Glycerol
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/3000 - 1/8000. Predicted molecular weight: 34 kDa.
ELISA 1/5000 - 1/10000.

Target

  • RelevanceAntigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.
  • Alternative names
    • 30 kDa extracellular protein antibody
    • Antigen 85 complex B antibody
    • Mycobacterium antibody
    • Mycolyl transferase 85B antibody

Anti-Mycobacterium tuberculosis Ag85B antibody images

  • All lanes : Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) at 1/5000 dilution

    Lane 1 : E coli, expressing his-tagged Ag85b
    Lane 2 : E coli, expressing his-tagged Ag85b

    Secondary
    Lane 1 : HRP conjugated goat anti-rabbit IgG
    Lane 2 : HRP conjugated goat anti-rat IgG


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 28 kDa (possible non-specific binding,degradation product).

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019)

This product has been referenced in:
  • Torabi-Parizi P  et al. Pathogen-related differences in the abundance of presented antigen are reflected in CD4+ T cell dynamic behavior and effector function in the lung. J Immunol 192:1651-60 (2014). Read more (PubMed: 24431231) »
  • Rath P  et al. Genetic regulation of vesiculogenesis and immunomodulation in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 110:E4790-7 (2013). WB . Read more (PubMed: 24248369) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
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Abcam user community

Verified customer

Submitted Apr 10 2013

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

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Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Thank you for contacting us. Yes, the lab has confirmed that the immunogen for this antibody was His-tagged. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Purified protein (transformed E coli)
Specification transformed E coli
Blocking step fish gelatin as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type Direct
Username

Abcam user community

Verified customer

Submitted Aug 29 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (Transformed E coli, expressing his-tagged Ag85b)
Loading amount 1.5 µg
Specification Transformed E coli, expressing his-tagged Ag85b
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"