Anti-Mycobacterium tuberculosis antibody (Biotin) (ab20027)

Overview

  • Product name
    Anti-Mycobacterium tuberculosis antibody (Biotin)
    See all Mycobacterium tuberculosis primary antibodies
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Minimum of two major M. tuberculosis bands by immunoelectrophoresis (gamma & beta).
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with Mycobacteria species including M. avium, M. phlei and M. parafortuitum. Does not react with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. Not yet tested in other species.
  • Immunogen

    Purified Protein Derivative (used for Tb skin testing)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Do not perform antigen retrieval.

Target

  • Relevance
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names
    • M. tuberculosis antibody

References for Anti-Mycobacterium tuberculosis antibody (Biotin) (ab20027)

ab20027 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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